Difference between revisions of "Part:BBa K3013003"

Revision as of 11:32, 19 October 2019

mamC-FbFP BS2 fusion

This fusion protein consists of mamC a glycine linker and a fluorescent protein. MamC is suggested to be located in the magnetosome membrane, wich are produced by magnetotactic bacteria. To report expression of mamC FbFP BS2 is used. This part is an improvement of part BBa_K1094401, because magnetotacic bacteria will produce magnetozomes under anaerobic conditions. In this case there is the need of an anaerobic reporter, as eGFP is dependent to oxygen to develope fluorescence. FbFP BS2 (BBa_K3013002) is flavin mononucleotide-based an independent to oxygen. The glycine linker will help the correct folding of mamC and FbFP BS2.

Usage and Biology

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 498
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 169
Illegal NgoMIV site found at 253
Illegal AgeI site found at 666
1000
COMPATIBLE WITH RFC[1000]

Revision as of 11:31, 19 October 2019 (view source) Gereon (Talk \| contribs) m ← Older edit		Revision as of 11:32, 19 October 2019 (view source) Gereon (Talk \| contribs) m Newer edit →
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	<partinfo>BBa_K3013003 short</partinfo>		<partinfo>BBa_K3013003 short</partinfo>

−	This fusion protein consists of mamC a glycine linker and a fluorescent protein. MamC is suggested to be located in the magnetosome membrane, wich are produced by magnetotactic bacteria. To report expression of mamC FbFP BS2 is used. This part is an improvement of part <partinfo>BBa_K1094401</partinfo>, because magnetotacic bacteria will produce magnetozomes under anaerobic conditions. In this case there is the need of an anaerobic reporter, as eGFP is dependent to oxygen to develope fluorescence. FbFP BS2 (BBa_K3013002) is flavin mononucleotide-based an independent to oxygen. The glycine linker will help the correct folding of mamC and FbFP BS2.	+	This fusion protein consists of mamC a glycine linker and a fluorescent protein. MamC is suggested to be located in the magnetosome membrane, wich are produced by magnetotactic bacteria. To report expression of mamC FbFP BS2 is used. This part is an improvement of part <partinfo>BBa_K1094401</partinfo>, because magnetotacic bacteria will produce magnetozomes under anaerobic conditions. In this case there is the need of an anaerobic reporter, as eGFP is dependent to oxygen to develope fluorescence. FbFP BS2 (<partinfo>BBa_K3013002</partinfo>) is flavin mononucleotide-based an independent to oxygen. The glycine linker will help the correct folding of mamC and FbFP BS2.