Difference between revisions of "Part:BBa K3013002:Design"

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===Design Notes===
 
===Design Notes===
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We optimized the sequence for <i>E. coli</i>.
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We optimized the sequence for <i>E. coli</i>. This part does not contain a start codon, as it is designed for a fusion protein. If you want to use this part alone, consider adding ATG!
  
 
===Source===
 
===Source===

Latest revision as of 11:25, 19 October 2019


FbFP BS2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 87
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 255
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We optimized the sequence for E. coli. This part does not contain a start codon, as it is designed for a fusion protein. If you want to use this part alone, consider adding ATG!

Source

We’ve got the sequence of this part from the additional data (https://www.ncbi.nlm.nih.gov/nuccore/EF418615) of the following paper [Drepper T, Eggert T, Circolone F, Heck A, Krauss U, Guterl JK, Wendorff M, Losi A, Gartner W, Jaeger KE: Reporter proteins for in vivo fluorescence without oxygen. Nat Biotechnol. 2007, 25: 443-445. 10.1038/nbt1293.].

References