Coding

Part:BBa_K2960011

Designed by: Ann Metzloff   Group: iGEM19_Cornell   (2019-10-16)


The mlrF enzyme

A cassette of enzymes endogenous to Sphingopyxis sp. has been shown to sequentially break down microcystin-LR (the most common and toxic microcystin that results from harmful algal blooms). The enzymes have been named mlrA, mlrB, mlrC, mlrD, mlrE, and mlrF.

MlrF has recently been identified in the genome of Sphingopyxis sp. strain C-1 and may be involved in microcystin degradation; is the expected sixth enzyme in the microcystin-degradation pathway. Its exact function is still unknown but it likely is involved with catalyzing reactions downstream to those of mlrA through C.


Figure 1. Schematic of the sequential degradation of MC-LR by the mlr enzyme cluster. MlrF is circled in red.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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