Part:BBa_K2960010

The mlrE enzyme

A cassette of enzymes endogenous to Sphingopyxis sp. has been shown to sequentially break down microcystin-LR (the most common and toxic microcystin that results from harmful algal blooms). The enzymes have been named mlrA, mlrB, mlrC, mlrD, mlrE, and mlrF.

MlrE has recently been identified in the genome of Sphingopyxis sp. strain C-1 and may be involved in microcystin degradation. Its exact function is still unknown. The gene for mlrE is, however, close in proximity to the gene for mlrB, so it is hypothesized that mlrE enhances the function of mlrB (or vice versa).

Figure 1. Schematic of the sequential degradation of MC-LR by the mlr enzyme cluster. MlrE is circled in red.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1125
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 84
  
• 1000
  COMPATIBLE WITH RFC[1000]