Difference between revisions of "Part:BBa K2771003:Experience"
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<h2>USP-Brazil Characterization</h2> | <h2>USP-Brazil Characterization</h2> | ||
| − | This part has shown to work when in the context of expressing the reporter [https://parts.igem.org/Part:BBa_K2771020 BBa_K2771020] and co-transformed with the part [https://parts.igem.org/Part:BBa_K2771030 BBa_K2771030]. In an assay in 2mL of LB in a 24-well plate, cells were grown overnight, then diluted 1:10 and grown for 1h. This was followed by induction of the system by arabinose 2.5mM to kickstart HSL production by LuxI. At evety chosen timepoint, samples were centrifuged and ressuspended in H2O. Excitation-emission wavelenghts for fluorescence measurement were 430-480 for ECFP and 500-530 for EYFP. Data in the graph is for promoter activity expressing EYFP and having this fluorescence divided by the constitutive ECFP fluorescence measurement: | + | This part has shown to somewhat work when in the context of expressing the reporter [https://parts.igem.org/Part:BBa_K2771020 BBa_K2771020] and co-transformed with the part [https://parts.igem.org/Part:BBa_K2771030 BBa_K2771030]. In an assay in 2mL of LB in a 24-well plate, cells were grown overnight, then diluted 1:10 and grown for 1h. This was followed by induction of the system by arabinose 2.5mM to kickstart HSL production by LuxI. At evety chosen timepoint, samples were centrifuged and ressuspended in H2O. Excitation-emission wavelenghts for fluorescence measurement were 430-480 for ECFP and 500-530 for EYFP. Data in the graph is for promoter activity expressing EYFP and having this fluorescence divided by the constitutive ECFP fluorescence measurement: |
[[File:T--USP-Brazil--Others assay.png|500px]] | [[File:T--USP-Brazil--Others assay.png|500px]] | ||
Latest revision as of 01:25, 18 October 2018
USP-Brazil Characterization
This part has shown to somewhat work when in the context of expressing the reporter BBa_K2771020 and co-transformed with the part BBa_K2771030. In an assay in 2mL of LB in a 24-well plate, cells were grown overnight, then diluted 1:10 and grown for 1h. This was followed by induction of the system by arabinose 2.5mM to kickstart HSL production by LuxI. At evety chosen timepoint, samples were centrifuged and ressuspended in H2O. Excitation-emission wavelenghts for fluorescence measurement were 430-480 for ECFP and 500-530 for EYFP. Data in the graph is for promoter activity expressing EYFP and having this fluorescence divided by the constitutive ECFP fluorescence measurement:
Applications of BBa_K2771003
User Reviews
UNIQ4344212ef5f8cb0c-partinfo-00000000-QINU UNIQ4344212ef5f8cb0c-partinfo-00000001-QINU