Difference between revisions of "Part:BBa K2660001"
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<partinfo>BBa_K2660001 short</partinfo>
 
<partinfo>BBa_K2660001 short</partinfo>
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GFP with his tag for purification under strong promoter T7. This part has the cell-penetrating peptide (CPP) penetratin (<a href="https://parts.igem.org/Part:BBa_K2660000">BBa_K2660000</a>) and was used by iGEM Unesp Brazil 2018 Team to evaluate this CPP in Caco-2 cell culture. We used E.coli BL21 (DE3) for expression of this part: addition of 1mM IPTG leads to expression of the T7 DNA polymerase which in turn drives expression of GFP.  
 
GFP with his tag for purification under strong promoter T7. This part has the cell-penetrating peptide (CPP) penetratin (<a href="https://parts.igem.org/Part:BBa_K2660000">BBa_K2660000</a>) and was used by iGEM Unesp Brazil 2018 Team to evaluate this CPP in Caco-2 cell culture. We used E.coli BL21 (DE3) for expression of this part: addition of 1mM IPTG leads to expression of the T7 DNA polymerase which in turn drives expression of GFP.  
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<!-- Add more about the biology of this part here
 
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===Usage and Biology===
 
===Usage and Biology===

Revision as of 23:38, 12 October 2018


T7 promoter driving 6-his tagged GFP with penetratin GFP with his tag for purification under strong promoter T7. This part has the cell-penetrating peptide (CPP) penetratin (BBa_K2660000) and was used by iGEM Unesp Brazil 2018 Team to evaluate this CPP in Caco-2 cell culture. We used E.coli BL21 (DE3) for expression of this part: addition of 1mM IPTG leads to expression of the T7 DNA polymerase which in turn drives expression of GFP. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 750