Difference between revisions of "Part:BBa K2660001"
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<partinfo>BBa_K2660001 short</partinfo> | <partinfo>BBa_K2660001 short</partinfo> | ||
| − | GFP with his tag for purification under strong promoter T7. This part has the cell-penetrating peptide (CPP) penetratin and was used by iGEM Unesp Brazil 2018 Team to evaluate this CPP in Caco-2 cell culture. We used E.coli BL21 (DE3) for expression of this part: addition of 1mM IPTG leads to expression of the T7 DNA polymerase which in turn drives expression of GFP. | + | GFP with his tag for purification under strong promoter T7. This part has the cell-penetrating peptide (CPP) penetratin (<a href="https://parts.igem.org/Part:BBa_K2660000">BBa_K2660000</a>) and was used by iGEM Unesp Brazil 2018 Team to evaluate this CPP in Caco-2 cell culture. We used E.coli BL21 (DE3) for expression of this part: addition of 1mM IPTG leads to expression of the T7 DNA polymerase which in turn drives expression of GFP. |
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Revision as of 23:37, 12 October 2018
T7 promoter driving 6-his tagged GFP with penetratin
GFP with his tag for purification under strong promoter T7. This part has the cell-penetrating peptide (CPP) penetratin (<a href="https://parts.igem.org/Part:BBa_K2660000">BBa_K2660000</a>) and was used by iGEM Unesp Brazil 2018 Team to evaluate this CPP in Caco-2 cell culture. We used E.coli BL21 (DE3) for expression of this part: addition of 1mM IPTG leads to expression of the T7 DNA polymerase which in turn drives expression of GFP.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 750