Difference between revisions of "Part:BBa K2656025"

Latest revision as of 03:20, 18 October 2018

BSMT1 Coding Sequence

Figure 1. DNA basic parts domestication. Third construction refers to CDS GB domestication.

Part BBa_K2656025 is the BMSTI coding sequence BBa_J45004 compatible with both Biobrick and [http://2018.igem.org/Team:Valencia_UPV/Design GoldenBraid 3.0] assembly methods. The protein encoded in this CDS catalyzes the conversion of benzoic acid to methyl benzoate and salicylic acid to methyl salicylate. Methyl benzoate has a mint-like smell. It can be combined with other compatible parts from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Valencia UPV IGEM 2018 Printeria Collection] to assemble transcriptional units with the Golden Gate assembly protocol .

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 664
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 __NOTOC__
 <partinfo>BBa_K2656025 short</partinfo>
+[[File:T--Valencia_UPV--im11UPV2018.png|250px|thumb|right|alt=domestication.|Figure 1. DNA basic parts domestication. Third construction refers to CDS GB domestication. ]]
 Part BBa_K2656025 is the BMSTI coding sequence [https://parts.igem.org/Part:BBa_J45004  BBa_J45004] compatible with both Biobrick and [http://2018.igem.org/Team:Valencia_UPV/Design GoldenBraid 3.0] assembly methods. The protein encoded in this CDS catalyzes the conversion of benzoic acid to methyl benzoate and salicylic acid to methyl salicylate. Methyl benzoate has a mint-like smell.
 It can be combined with other compatible parts from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Valencia UPV IGEM 2018 Printeria Collection] to assemble transcriptional units with the <html><a href="https://static.igem.org/mediawiki/parts/b/bc/T--Valencia_UPV--protocols.pdf#page=27"> Golden Gate assembly protocol </a></html>.