Difference between revisions of "Part:BBa K2656024"
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The LVA tag causes an increase in protease activity and, therefore, a faster degradation of the reporter protein in the cell. | The LVA tag causes an increase in protease activity and, therefore, a faster degradation of the reporter protein in the cell. | ||
| − | It can be combined with other compatible parts from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Valencia UPV IGEM 2018 Printeria Collection] to assemble transcriptional units in a BsaI one-step reaction with the | + | It can be combined with other compatible parts from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Valencia UPV IGEM 2018 Printeria Collection] to assemble transcriptional units in a BsaI one-step reaction with the <html><a href="https://static.igem.org/mediawiki/parts/b/bc/T--Valencia_UPV--protocols.pdf#page=27"> Golden Gate assembly protocol </a></html>. |
Latest revision as of 00:32, 18 October 2018
mRFP1_LVA Coding Sequence
Part BBa_K2656024 is the Monomeric red fluorescent protein with LVA degradation tag BBa_K1399001 adapted into the Golden Gate assembly method. Thus, it is a basic part compatible with both Biobrick and [http://2018.igem.org/Team:Valencia_UPV/Design GoldenBraid 3.0] grammar.
The LVA tag causes an increase in protease activity and, therefore, a faster degradation of the reporter protein in the cell.
It can be combined with other compatible parts from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Valencia UPV IGEM 2018 Printeria Collection] to assemble transcriptional units in a BsaI one-step reaction with the Golden Gate assembly protocol .
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 556
Illegal AgeI site found at 668 - 1000COMPATIBLE WITH RFC[1000]