Difference between revisions of "Part:BBa K2656023"

Revision as of 00:31, 18 October 2018

GFPmut3b_LVA Coding Sequence

Part BBa_K2656013 is the GFPmut3b coding sequence with the LVA degradation tag. It is the BBa_K1399004 original sequence adapted to be Golden Gate compatible. Thus, it can be used with Biobrick and [http://2018.igem.org/Team:Valencia_UPV/Design Golden Braid] assembly methods. The LVA tag causes an increase in protease activity and, therefore, a faster degradation of the reporter protein in the cell.

It can be combined with other compatible parts from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Valencia UPV IGEM 2018 Printeria Collection] to assemble transcriptional units with a BsaI one-step reaction with the Golden Gate assembly protocol .

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 The LVA tag causes an increase in protease activity and, therefore, a faster degradation of the reporter protein in the cell.
-It can be combined with other compatible parts from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Valencia UPV IGEM 2018 Printeria Collection] to assemble transcriptional units with a BsaI one-step reaction with the [http://2018.igem.org/Team:Valencia_UPV/Protocols Golden Gate assembly protocol].
+It can be combined with other compatible parts from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Valencia UPV IGEM 2018 Printeria Collection] to assemble transcriptional units with a BsaI one-step reaction with the <html><a href="https://static.igem.org/mediawiki/parts/b/bc/T--Valencia_UPV--protocols.pdf#page=27"> Golden Gate assembly protocol </a></html>.
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