Difference between revisions of "Part:BBa K2656006"

Latest revision as of 01:16, 18 October 2018

Minimal pBAD promoter

Figure 1. DNA basic parts domestication. First construction refers to promoter GB domestication.

Part BBa_K2656006 is the promoter BBa_K2442101 compatible with both Biobrick and [http://2018.igem.org/Team:Valencia_UPV/Design GoldenBraid 3.0] assembly methods. This promoter is negatively regulated by AraC in the ausence of L-arabinose. It can be combined with other compatible parts from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Valencia UPV IGEM 2018 Printeria Collection] to assemble transcriptional units with the Golden Gate assembly protocol .

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 246
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 81
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 63

@@ Line 2: / Line 2: @@
 __NOTOC__
 <partinfo>BBa_K2656006 short</partinfo>
+[[File:T--Valencia_UPV--im11UPV2018.png|250px|thumb|centro|alt=domestication.|Figure 1. DNA basic parts domestication. First construction refers to promoter GB domestication. ]]
-Part BBa_K2656006 is the promoter [https://parts.igem.org/Part:BBa_K2442101  BBa_K2442101] compatible with both Biobrick and [http://2018.igem.org/Team:Valencia_UPV/Design GoldenBraid 3.0] assembly methods. This promoter is positively regulated by AraC in the presence of L-arabinose. It can be combined with other compatible parts from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Valencia UPV IGEM 2018 Printeria Collection] to assemble transcriptional units with the <html><a href="https://static.igem.org/mediawiki/parts/b/bc/T--Valencia_UPV--protocols.pdf#page=27"> Golden Gate assembly protocol </a></html>.
+Part BBa_K2656006 is the promoter [https://parts.igem.org/Part:BBa_K2442101  BBa_K2442101] compatible with both Biobrick and [http://2018.igem.org/Team:Valencia_UPV/Design GoldenBraid 3.0] assembly methods. This promoter is negatively regulated by AraC in the ausence of L-arabinose. It can be combined with other compatible parts from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Valencia UPV IGEM 2018 Printeria Collection] to assemble transcriptional units with the <html><a href="https://static.igem.org/mediawiki/parts/b/bc/T--Valencia_UPV--protocols.pdf#page=27"> Golden Gate assembly protocol </a></html>.
 <!-- Add more about the biology of this part here