Difference between revisions of "Part:BBa K2656000"
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<partinfo>BBa_K2656000 short</partinfo> | <partinfo>BBa_K2656000 short</partinfo> | ||
+ | [[File:T--Valencia_UPV--im11UPV2018.png|250px|thumb|centro|alt=domestication.|Figure 1. DNA basic parts domestication. First construction refers to promoter GB domestication. ]] | ||
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Part BBa_K2656000 is the constitutive promoter derived from the T7 bacteriophage that allows high expression of proteins in the presence of the T7 DNA polymerase [https://parts.igem.org/Part:BBa_I719005 BBa_I719005 ] compatible with both Biobrick and [http://2018.igem.org/Team:Valencia_UPV/Design GoldenBraid 3.0] assembly methods. It can be combined with other compatible parts from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Valencia UPV IGEM 2018 Printeria Collection] to assemble transcriptional units with the <html><a href="https://static.igem.org/mediawiki/parts/b/bc/T--Valencia_UPV--protocols.pdf#page=27"> Golden Gate assembly protocol </a></html>. | Part BBa_K2656000 is the constitutive promoter derived from the T7 bacteriophage that allows high expression of proteins in the presence of the T7 DNA polymerase [https://parts.igem.org/Part:BBa_I719005 BBa_I719005 ] compatible with both Biobrick and [http://2018.igem.org/Team:Valencia_UPV/Design GoldenBraid 3.0] assembly methods. It can be combined with other compatible parts from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Valencia UPV IGEM 2018 Printeria Collection] to assemble transcriptional units with the <html><a href="https://static.igem.org/mediawiki/parts/b/bc/T--Valencia_UPV--protocols.pdf#page=27"> Golden Gate assembly protocol </a></html>. |
Revision as of 01:05, 18 October 2018
T7 phage strong promoter
Part BBa_K2656000 is the constitutive promoter derived from the T7 bacteriophage that allows high expression of proteins in the presence of the T7 DNA polymerase BBa_I719005 compatible with both Biobrick and [http://2018.igem.org/Team:Valencia_UPV/Design GoldenBraid 3.0] assembly methods. It can be combined with other compatible parts from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Valencia UPV IGEM 2018 Printeria Collection] to assemble transcriptional units with the Golden Gate assembly protocol .
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]