Difference between revisions of "Part:BBa K2611010"

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<partinfo>BBa_K2611010 short</partinfo>
 
<partinfo>BBa_K2611010 short</partinfo>
  
We added a spacer sequence and a NGG site closely before the promoter (<a href="https://parts.igem.org/Part:BBa_J23101">J23101</a>). This spacer sequence can be recognized by the sgRNA we designed to direct dCas9. Once dCas9 binds with the spacer sequence, the expression of GFP will be repressed.We have submitted the sgRNA part (BBa_K2611000) and spacer-GFP part(BBa_K2611001). In this composite part, we linked them together.We observed positive colonies transformed with sgRNA(spacer J23101-GFP)-spacer J23101-GFP(part BBa_K2611010) under a stereo fluorescence microscope.Green fluorescence was observed.(Fig.1)
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We added a spacer sequence and a NGG site closely before the promoter (<a href='https://parts.igem.org/Part:BBa_J23101'>J23101</a>). This spacer sequence can be recognized by the sgRNA we designed to direct dCas9. Once dCas9 binds with the spacer sequence, the expression of GFP will be repressed.We have submitted the sgRNA part (BBa_K2611000) and spacer-GFP part(BBa_K2611001). In this composite part, we linked them together.We observed positive colonies transformed with sgRNA(spacer J23101-GFP)-spacer J23101-GFP(part BBa_K2611010) under a stereo fluorescence microscope.Green fluorescence was observed.(Fig.1)
  
 
[[File:Long description.png]]
 
[[File:Long description.png]]

Revision as of 07:56, 17 October 2018


sgRNA(spacer J23101-GFP)-spacer J23101-GFP

We added a spacer sequence and a NGG site closely before the promoter (<a href='https://parts.igem.org/Part:BBa_J23101'>J23101</a>). This spacer sequence can be recognized by the sgRNA we designed to direct dCas9. Once dCas9 binds with the spacer sequence, the expression of GFP will be repressed.We have submitted the sgRNA part (BBa_K2611000) and spacer-GFP part(BBa_K2611001). In this composite part, we linked them together.We observed positive colonies transformed with sgRNA(spacer J23101-GFP)-spacer J23101-GFP(part BBa_K2611010) under a stereo fluorescence microscope.Green fluorescence was observed.(Fig.1)

Long description.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 226
    Illegal NheI site found at 249
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 924