Difference between revisions of "Part:BBa K2586003"

Revision as of 10:18, 11 September 2018

aroE: 3-phosphoshikimate 1-carboxyvinyltransferase

This part encodes for the 3-phosphoshikimate 1-carboxyvinyltransferase.
The encoded enzyme is needed for the biosynthesis of essential aromatic amino acids in the shikimate pathway. It is essential in Bacillus subtilis , as three essential aromatic amino acids are products of the pathway: Trp, Phe and Tyr.
aroE encodes the 5-enolpyruvylshikimate-3-phosphate (EPSP) synthase, which is the main target of the herbicide glyphosate and is strongly inhibited through glyphosate application. <img src="" height="100" width="200" alt="CHIP-Logo" border="2" align="right" hspace="50" vspace="50"> An amplification of aroE allows for glyphosate resistance (1), this is used in multiple GM plants to increase the yield and improve farming applications.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 90
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 415
1000
COMPATIBLE WITH RFC[1000]

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 The encoded enzyme is needed for the biosynthesis of essential aromatic amino acids in the shikimate pathway. It is essential in <i>Bacillus subtilis </i>, as three essential aromatic amino acids are products of the pathway: Trp, Phe and Tyr. <br/>
 <i>aroE</i> encodes the 5-enolpyruvylshikimate-3-phosphate (EPSP) synthase, which is the main target of the herbicide glyphosate and is strongly inhibited through glyphosate application.
+<img src="https://static.igem.org/mediawiki/2018/a/a9/T--Goettingen--shikimate_pathway_ordinary.png" height="100" width="200" alt="CHIP-Logo" border="2" align="right" hspace="50" vspace="50">
 An amplification of <i>aroE</i> allows for glyphosate resistance (1), this is used in multiple GM plants to increase the yield and improve farming applications.