Difference between revisions of "Part:BBa K2560127:Design"
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===Design Notes=== | ===Design Notes=== | ||
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+ | This part was created by polymerase chain reaction (PCR) on the template pTrc-McrCa and subsequent integration into the part entry vector <a href="https://parts.igem.org/Part:BBa_K2560002">BBa_K2560002</a> using Golden Gate assembly. | ||
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+ | <b> Forward oligo:</b> | ||
+ | TTCGTCTCCCTCATGTTTTCAAATATGTATCCGCTCATGAGAC | ||
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+ | <b> Reverse Oligo:</b> | ||
+ | TTCGTCTCCCGTGACCCACGCTCACC | ||
===Source=== | ===Source=== | ||
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+ | As Source DNA we used pTrc-McrCa from the Erb Lab in Marburg, Germany. | ||
− | + | </html> |
Latest revision as of 23:51, 17 October 2018
Phytobrick version of Carbenicillin Resistance in BBa_K2560002
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part was created by polymerase chain reaction (PCR) on the template pTrc-McrCa and subsequent integration into the part entry vector BBa_K2560002 using Golden Gate assembly.
Forward oligo: TTCGTCTCCCTCATGTTTTCAAATATGTATCCGCTCATGAGAC
Reverse Oligo: TTCGTCTCCCGTGACCCACGCTCACC
Source
As Source DNA we used pTrc-McrCa from the Erb Lab in Marburg, Germany.