Difference between revisions of "Part:BBa K2525018"
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This part contains RBS (B0032), SrpR (BBa_K1725060) and Terminator (BBa_K1725081). The SrpR repressor has higher cooperativity (n = 3.2) which negatively regulates (represses) promoter pSrpR. | This part contains RBS (B0032), SrpR (BBa_K1725060) and Terminator (BBa_K1725081). The SrpR repressor has higher cooperativity (n = 3.2) which negatively regulates (represses) promoter pSrpR. | ||
− | This can be used as a part of a genetic circuit, where the SrpR repressor is present. The high cooperativity of this part is of great significance to synthetic biology, as it can be used to give steeper response curves for repression. Ideally, when we tend to look at genetic elements as digital logic tools, we require steep transitions of protein levels from 0 to 1 or 1 to 0 (representing and ON and OFF state). However, due to various limitations in biological systems, the response is often in the form of a gradient and not a steep curve. Due to its high cooperativity, | + | This can be used as a part of a genetic circuit, where the SrpR repressor is present. The high cooperativity of this part is of great significance to synthetic biology, as it can be used to give steeper response curves for repression. Ideally, when we tend to look at genetic elements as digital logic tools, we require steep transitions of protein levels from 0 to 1 or 1 to 0 (representing and ON and OFF state). However, due to various limitations in biological systems, the response is often in the form of a gradient and not a steep curve. Due to its high cooperativity, SrpR can give a much better and steeper curve than most traditional repressors. This is highly desirable. |
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Latest revision as of 23:28, 27 October 2017
RBS + SrpR + T
This part contains RBS (B0032), SrpR (BBa_K1725060) and Terminator (BBa_K1725081). The SrpR repressor has higher cooperativity (n = 3.2) which negatively regulates (represses) promoter pSrpR.
This can be used as a part of a genetic circuit, where the SrpR repressor is present. The high cooperativity of this part is of great significance to synthetic biology, as it can be used to give steeper response curves for repression. Ideally, when we tend to look at genetic elements as digital logic tools, we require steep transitions of protein levels from 0 to 1 or 1 to 0 (representing and ON and OFF state). However, due to various limitations in biological systems, the response is often in the form of a gradient and not a steep curve. Due to its high cooperativity, SrpR can give a much better and steeper curve than most traditional repressors. This is highly desirable.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 509
- 1000COMPATIBLE WITH RFC[1000]