Difference between revisions of "Part:BBa K2460011"

 
 
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<partinfo>BBa_K2460011 short</partinfo>
 
<partinfo>BBa_K2460011 short</partinfo>
  
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This part is improved from [[Part:BBa_K1039000|BBa_K1039000]].
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This is a recombination site attP for phiC31 integrase ([[Part:BBa_K1039012|BBa_K1039012]]). When used with phiC31 attB ([[Part:BBa_K2460010|BBa_K2460010]]) and phiC31 integrase, it can flip a DNA sequence flanked by attB and attP sites, as well as integrating a circular DNA with a attB/P site into a linear/circular DNA with a attP/B site.
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For more information about kinetic characterization and the orthogonality between the integrase and some other serine recombinase, go to [[Part:BBa_K1039012|phiC31 integrase]].
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 03:01, 2 November 2017


φC31 attP

This part is improved from BBa_K1039000.

This is a recombination site attP for phiC31 integrase (BBa_K1039012). When used with phiC31 attB (BBa_K2460010) and phiC31 integrase, it can flip a DNA sequence flanked by attB and attP sites, as well as integrating a circular DNA with a attB/P site into a linear/circular DNA with a attP/B site.

For more information about kinetic characterization and the orthogonality between the integrase and some other serine recombinase, go to phiC31 integrase.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]