Difference between revisions of "Part:BBa K2460008"

 
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<partinfo>BBa_K2460008 short</partinfo>
 
<partinfo>BBa_K2460008 short</partinfo>
  
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This is a recombination site attB for TG1 integrase ([[Part:BBa_K2460007|BBa_K2460007]]). When used with phiBT1 attP ([[Part:BBa_K2460009|BBa_K2460009]]) and phiBT1 integrase, it can flip a DNA sequence flanked by attB and attP sites, as well as integrating a circular DNA with a attB/P site into a linear/circular DNA with a attP/B site.
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For more information about kinetic characterization and the orthogonality between the integrase and some other serine recombinase, go to [[Part:BBa_K2460007|TG1 integrase]].
  
 
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Revision as of 02:40, 2 November 2017


TG1 attB

This is a recombination site attB for TG1 integrase (BBa_K2460007). When used with phiBT1 attP (BBa_K2460009) and phiBT1 integrase, it can flip a DNA sequence flanked by attB and attP sites, as well as integrating a circular DNA with a attB/P site into a linear/circular DNA with a attP/B site. For more information about kinetic characterization and the orthogonality between the integrase and some other serine recombinase, go to TG1 integrase.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]