Difference between revisions of "Part:BBa K2460006"

 
Line 3: Line 3:
 
<partinfo>BBa_K2460006 short</partinfo>
 
<partinfo>BBa_K2460006 short</partinfo>
  
1
+
This is a recombination site attP for phiRv1 integrase ([[Part:BBa_K2460004|BBa_K2460004]]). When used with phiBT1 attB ([[Part:BBa_K2460005|BBa_K2460005]]) and phiBT1 integrase, it can flip a DNA sequence flanked by attB and attP sites, as well as integrating a circular DNA with a attB/P site into a linear/circular DNA with a attP/B site.
 +
For more information about kinetic characterization and the orthogonality between the integrase and some other serine recombinase, go to [[Part:BBa_K2460004|phiRv1 integrase]].
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 02:38, 2 November 2017


φRv1 attP

This is a recombination site attP for phiRv1 integrase (BBa_K2460004). When used with phiBT1 attB (BBa_K2460005) and phiBT1 integrase, it can flip a DNA sequence flanked by attB and attP sites, as well as integrating a circular DNA with a attB/P site into a linear/circular DNA with a attP/B site. For more information about kinetic characterization and the orthogonality between the integrase and some other serine recombinase, go to phiRv1 integrase.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]