Difference between revisions of "Part:BBa K2460005"

Latest revision as of 02:47, 2 November 2017

φRv1 attB

This is a recombination site attB for phiRv1 integrase (BBa_K2460004). When used with phiBT1 attP (BBa_K2460006) and phiRv1 integrase, it can flip a DNA sequence flanked by attB and attP sites, as well as integrating a circular DNA with a attB/P site into a linear/circular DNA with a attP/B site. For more information about kinetic characterization and the orthogonality between the integrase and some other serine recombinase, go to phiRv1 integrase.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2460005 short</partinfo>
-This is a recombination site attB for phiRv1 integrase ([[Part:BBa_K2460004|BBa_K2460004]]). When used with phiBT1 attP ([[Part:BBa_K2460006|BBa_K2460006]]) and phiBT1 integrase, it can flip a DNA sequence flanked by attB and attP sites, as well as integrating a circular DNA with a attB/P site into a linear/circular DNA with a attP/B site.
+This is a recombination site attB for phiRv1 integrase ([[Part:BBa_K2460004|BBa_K2460004]]). When used with phiBT1 attP ([[Part:BBa_K2460006|BBa_K2460006]]) and phiRv1 integrase, it can flip a DNA sequence flanked by attB and attP sites, as well as integrating a circular DNA with a attB/P site into a linear/circular DNA with a attP/B site.
 For more information about kinetic characterization and the orthogonality between the integrase and some other serine recombinase, go to [[Part:BBa_K2460004|phiRv1 integrase]].