Difference between revisions of "Part:BBa K2460002"

 
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<partinfo>BBa_K2460002 short</partinfo>
 
<partinfo>BBa_K2460002 short</partinfo>
  
This is a recombination site attB for phiBT1.
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This is a recombination site attB for phiBT1 integrase ([[Part:BBa_K2460001|BBa_K2460001]]). When used with phiBT1 attP ([[Part:BBa_K2460003|BBa_K2460003]]) and phiBT1 integrase, it can flip a DNA sequence flanked by attB and attP sites, as well as integrating a circular DNA with a attB/P site into a linear/circular DNA with a attP/B site.
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For more information about kinetic characterization and the orthogonality between the integrase and some other serine recombinase, go to [[Part:BBa_K2460001|phiBT1 integrase]].
  
 
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Latest revision as of 02:31, 2 November 2017


φBT1 attB

This is a recombination site attB for phiBT1 integrase (BBa_K2460001). When used with phiBT1 attP (BBa_K2460003) and phiBT1 integrase, it can flip a DNA sequence flanked by attB and attP sites, as well as integrating a circular DNA with a attB/P site into a linear/circular DNA with a attP/B site. For more information about kinetic characterization and the orthogonality between the integrase and some other serine recombinase, go to phiBT1 integrase.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]