Difference between revisions of "Part:BBa K2455005"

Latest revision as of 01:09, 2 November 2017

CPP-mTag BFP

This is an improved version of the Blue Fluorescent Protein (mTag BFP; BBa_K592100) carrying an N-terminal nona-arginin tag, and a C-terminal hexa-histidine tag. The R9-tag is a Cell-Penetrating Peptide giving its associated protein the ability to pass through plasma membranes.

For our study we demonstrated that this improved version of mTag BFP had fluorescence of similar levels to the original protein, and was able to stain both Escherichia coli and Pseudomonas putida cells, presumably through internalisation of the protein.

Previous studies have shown R9 associated proteins to be able to enter a variety of cell types (Chang et al., 2005).

DISCLAIMER: This biobrick carries an illegal EcoRI site partly in the end of the BFP and party in the beginning of the linker region - For data and design please see CPP-SYFP2.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 741
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 741
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 741
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 741
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 741
1000
COMPATIBLE WITH RFC[1000]

@@ Line 9: / Line 9: @@
 Previous studies have shown R9 associated proteins to be able to enter a variety of cell types ([https://academic.oup.com/pcp/article/46/3/482/1817347 Chang <i>et al., 2005</i>]).
-<b>DISCLAIMER:</b> This biobrick carries an illegal EcoRI site partly in the end of the BFP and party in the beginning of the linker region - For data and design please see [[Part:BBa_K2455002|R9-SYFP2-H6]].
+<b>DISCLAIMER:</b> This biobrick carries an illegal EcoRI site partly in the end of the BFP and party in the beginning of the linker region - For data and design please see [[Part:BBa_K2455002|CPP-SYFP2]].
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