Difference between revisions of "Part:BBa K2407000"
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<partinfo>BBa_K2407000 short</partinfo>
 
<partinfo>BBa_K2407000 short</partinfo>
  
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<p>This is a shortened <i>CUP1</i> promoter, improved from <partinfo>BBa_K2165004<partinfo>. This promoter is designed to be deleted with a few bases on the two ends. We retained 5 <i>ACE1/CUP1</i> binding sites, 2 TATA boxes, and one initiation element in the promoter. The complex of <i>ACE1</i> and copper ions will bind the promoter, which causes the activation of <i>CUP1</i> promoter with TATA boxes' help. In this way, this promoter played its key role with less bases.</p>
+
<p>This is a shortened <i>CUP1</i> promoter, improved from <partsinfo>BBa_K2165004<partsinfo>. This promoter is designed to be deleted with a few bases on the two ends. We retained 5 <i>ACE1/CUP1</i> binding sites, 2 TATA boxes, and one initiation element in the promoter. The complex of <i>ACE1</i> and copper ions will bind the promoter, which causes the activation of <i>CUP1</i> promoter with TATA boxes' help. In this way, this promoter played its key role with less bases.</p>
  
 
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Revision as of 10:29, 27 October 2017


Shortened CUP1 promoter without RFC sites

This is a shortened CUP1 promoter, improved from <partsinfo>BBa_K2165004<partsinfo>. This promoter is designed to be deleted with a few bases on the two ends. We retained 5 ACE1/CUP1 binding sites, 2 TATA boxes, and one initiation element in the promoter. The complex of ACE1 and copper ions will bind the promoter, which causes the activation of CUP1 promoter with TATA boxes' help. In this way, this promoter played its key role with less bases.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]