Difference between revisions of "Part:BBa K2270010"

Revision as of 03:03, 2 November 2017

superfolding GFP for Lactococcus lactis

Superfolding GFP codon-optimized for Bacillus subtilis. However, exhibits strong fluorescense signal in Lactococcus lactis. Used as protein report in L. lactis.

Usage and Biology

We (AQA_Unesp 2017) characterized the gene expression of sfGFP(Bs) during Lactococcus lactis growth under control of the usp45 promoter.

For more Details visit our [http://2017.igem.org/Team:AQA_Unesp/Results webpage]

Figure 1. Pellet of Lactococcus lactis cremoris cloned with Pusp45_GFP genes exited by a blue led transilluminator with filter.

Characterization

Figure 2. Growth curve and fluorescence from Lactococcus lactis cremoris cloned with Pusp45_GFP genes.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 13

@@ Line 5: / Line 5: @@
 Superfolding GFP codon-optimized for Bacillus subtilis. However, exhibits strong fluorescense signal in Lactococcus lactis. Used as protein report in L. lactis.
-<!-- Add more about the biology of this part here
+<!-- Add more about the biology of this part here-->
 ===Usage and Biology===
+We (AQA_Unesp 2017) characterized the gene expression of sfGFP(Bs)
+during Lactococcus lactis growth under control of the usp45 promoter.
+For more Details visit our [http://2017.igem.org/Team:AQA_Unesp/Results webpage]
+[[Image:AQA Unesp--l.latis-pusp45-gfp-pellet.png|thumb|right|400px| <p align="justify"> '''Figure 1. '''''Pellet of Lactococcus lactis cremoris cloned with Pusp45_GFP genes exited by a blue led transilluminator with filter.</p>]]
+===Characterization===
+[[Image:AQA Unesp--l.latis-pusp45-gfp.png|thumb|right|400px| <p align="justify"> '''Figure 2. '''''Growth curve and fluorescence from Lactococcus lactis cremoris cloned with Pusp45_GFP genes.</p>]]
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