Difference between revisions of "Part:BBa K2201027"

 
 
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<partinfo>BBa_K2201027 short</partinfo>
 
<partinfo>BBa_K2201027 short</partinfo>
  
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lacO_tight2 from E. coli BL21(DE3) and optimized
 
  
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===Usage and Biology===
 
===Usage and Biology===
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This part was used as a part of the retention and preservation system for unnatural base pairs. For a comprehensive characterization of <i>Pt</i>NTT2,
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please view <a href="https://parts.igem.org/Part:BBa_K2201004">BBa_K2201004</a>. A characterization of the lacO operon and Cas9 can be found here: <a href="https://parts.igem.org/Part:BBa_K2201020">BBa_K2201020</a> and <a href="https://parts.igem.org/Part:BBa_K2201023">BBa_K2201023</a>.
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</html>
  
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K2201027 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2201027 SequenceAndFeatures</partinfo>

Latest revision as of 14:18, 1 November 2017


PtNTT2(31-575)-lacO-Cas9 construct



Usage and Biology

This part was used as a part of the retention and preservation system for unnatural base pairs. For a comprehensive characterization of PtNTT2, please view BBa_K2201004. A characterization of the lacO operon and Cas9 can be found here: BBa_K2201020 and BBa_K2201023.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3269
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3269
    Illegal NheI site found at 3028
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3269
    Illegal BamHI site found at 5307
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3269
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3269
  • 1000
    COMPATIBLE WITH RFC[1000]