Difference between revisions of "Part:BBa K2165004"

Revision as of 01:57, 27 October 2017

CUP1 yeast inducible promoter with RFC[10] restriction sites removed

The Cup1 promoter enables binding of RNA Polymerase II and the subsequent transcription of downstream DNA to mRNA. It is activated by ACE1, a transcription factor which binds to copper ions. It was previously available as a standalone part as BBa_K945002, produced by Tec-Monterrery’s 2012 iGEM team; however, the original part contained numerous illegal restriction sites preventing its usability in standard biobrick assembly. Hard information of the Greenfield 2011 team’s BBa_K586000 claims the part is the Cup1 regulatory region, but based on the sequence and its twin we believe it to be mislabeled and that it is instead the CupI CDS.

We acquired our starting sequence from the Vicker’s Lab at the Australian Institute for Bioengineering and Nanotechnology via Addgene, subsequently removed or altered bases corresponding to illegal cut sites, added a biobrick prefix and suffix, and finally ordered the unit as a geneblock from IDT.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 8: / Line 8: @@
 <!-- Add more about the biology of this part here
-===Usage and Biology===
 The followings were edited by iGEM17_Tianjin
 <h2>Information Supplement</h2>
 <hr>
+<p>Transcription with <i>CUP1</i> promoter is activated by sequence-specific transcription factors which bind to the <i>CUP1</i> upstream activating sequence (<i>CUP1 UAS</i>). This sequence contains five binding sites for the Cu<sup>2+</sup>-dependent transcriptional activator <i>ACE1</i>/<i>CUP2</i>. The <i>CUP1 UAS</i> is necessary and sufficient for rapid and robust activation of this transcription.
+===Usage and Biology===