Difference between revisions of "Part:BBa K2113004"

 
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<partinfo>BBa_K2113004 parameters</partinfo>
 
<partinfo>BBa_K2113004 parameters</partinfo>
 
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===Mobyle@RPBS 3D Structure Prediction===
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(The following information has been contributed by SVCE_Chennai iGEM-2016)
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<span class='h3bb'><strong>3D Structure</strong></span>
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[[Image:MGG.gif]]
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===ProtParam Results===
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(The following information has been contributed by SVCE_Chennai iGEM-2016)
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Number of amino acids: 7
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Molecular weight: 1176.41
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Theoretical pI: 12.30
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Total number of negatively charged residues (Asp + Glu): 0
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Total number of positively charged residues (Arg + Lys): 3
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<strong>Atomic composition:</strong>
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<br>Carbon      (C)         56
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<br>Hydrogen    (H)         77
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<br>Nitrogen    (N)         19
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<br>Oxygen      (O)         8
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<br>Sulfur      (S)         1
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<br>Formula: C<sub>56</sub>H<sub>77</sub>N<sub>19</sub>O<sub>8</sub>S<sub>1</sub>
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<br>Total number of atoms: 161
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<br><strong>Extinction coefficients:</strong>
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<br>Extinction coefficients are in units of  M<sup>-1</sup> cm<sup>-1</sup>, at 280 nm measured in water.
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<br>Ext. coefficient:    16500
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<br>Abs 0.1% (=1 g/l):  14.026
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<br><strong>Estimated half-life:</strong>
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<br>The N-terminal of the sequence considered is M (Met).
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<br>The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
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<br>                            >20 hours (yeast, in vivo).
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<br>                            >10 hours (Escherichia coli, in vivo).
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<strong>Instability index:</strong>
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<br>The instability index (II) is computed to be 172.23
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<br>This classifies the protein as unstable.
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<br>Aliphatic index: 0.00
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<br>Grand average of hydropathicity (GRAVY): -2.043

Revision as of 07:14, 14 October 2016


AMP without glycine

It consists of cationic anti-microbial peptides (AMP) with consecutive sequences of arginine and tryptophan (WRWRWR).This can be used as an antimicrobial agent against a wide range of gram positive and gram negative bacteria.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



Mobyle@RPBS 3D Structure Prediction

(The following information has been contributed by SVCE_Chennai iGEM-2016)



3D Structure

MGG.gif


ProtParam Results

(The following information has been contributed by SVCE_Chennai iGEM-2016)

Number of amino acids: 7 Molecular weight: 1176.41 Theoretical pI: 12.30 Total number of negatively charged residues (Asp + Glu): 0 Total number of positively charged residues (Arg + Lys): 3


Atomic composition:


Carbon (C) 56
Hydrogen (H) 77
Nitrogen (N) 19
Oxygen (O) 8
Sulfur (S) 1



Formula: C56H77N19O8S1
Total number of atoms: 161



Extinction coefficients:


Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water.


Ext. coefficient: 16500
Abs 0.1% (=1 g/l): 14.026



Estimated half-life:


The N-terminal of the sequence considered is M (Met).


The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).


Instability index:


The instability index (II) is computed to be 172.23
This classifies the protein as unstable.



Aliphatic index: 0.00


Grand average of hydropathicity (GRAVY): -2.043