Difference between revisions of "Part:BBa K200030:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | Lipase A from B.subtilis | + | Lipase A was amplified from the B.subtilis genome 168 by polymerase chain reaction (PCR) using the following primers: |
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| + | Fwd = GCTCTAGAGATGCAAATGACGGAAGCTACCTG | ||
| + | Rev = CTGCAGCGGCCGCTACTAGTATTATTAACTTGATGTAATCATTGCG | ||
===Source=== | ===Source=== | ||
Latest revision as of 14:55, 20 September 2010
Lipase A from B.subtilis
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 726
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Lipase A was amplified from the B.subtilis genome 168 by polymerase chain reaction (PCR) using the following primers:
Fwd = GCTCTAGAGATGCAAATGACGGAAGCTACCTG Rev = CTGCAGCGGCCGCTACTAGTATTATTAACTTGATGTAATCATTGCG
Source
Lipase A from B.subtilis