The sequence codes for cellulose binding domain cbh2 in prokaryotic cells.
Contribution - iGEM 2017 SDSZ-China
Proof of the CBD function
The resulted solution after expression is mixed with a gauze piece, and the green color on gauze is recorded. Then the gauze is washed three times using ddH2O, but the green fluorescent on it is not significantly reduced, proving that the CDB is well functioned. In comparison, no green fluorescent is left after washing the gauze mixed with GFP-ChBD (Chintin binding domain).
[Fig. 1. GFP-CBD on gauze before washing]
[Fig. 2. GFP-CBD on gauze after washing]
[Fig. 3. GFP-ChBD on gauze before washing]
[Fig. 4. GFP-ChBD on gauze after washing]
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000Illegal SapI site found at 11