Difference between revisions of "Part:BBa K197010:Design"
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<partinfo>BBa_K197010 short</partinfo> | <partinfo>BBa_K197010 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
− | + | This part is in BglBricks Standard. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BglBricks Standard is available at: | |
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+ | [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BglBricks Standard Description Page] | ||
===Source=== | ===Source=== |
Latest revision as of 05:17, 22 October 2009
{ehaB}
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 589
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 589
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 589
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 589
Illegal AgeI site found at 132 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part is in BglBricks Standard. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BglBricks Standard is available at:
[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BglBricks Standard Description Page]
Source
PCR ca998/O09ig281 on M10052 (1229, EcoRI/BamHI) Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L) Product is pBca9523-O09ig281 {<ehaB>} ------------------------------------- Ca998 forward oligo gtatcacgaggcagaatttcag O09ig281 reverse oligo with stop codons removed for {<ehaB>} cggtaGGATCCccaggtatatttaacaccca