Difference between revisions of "Part:BBa K197005:Design"

 
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<partinfo>BBa_K197005 short</partinfo>
 
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===Design Notes===
 
===Design Notes===
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N/A
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This part is in BglBricks Standard.  It is flanked by BamHI and BglII sites instead of XbaI and SpeI.  More information about the BglBricks Standard is available at:
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[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BglBricks Standard Description Page]
  
 
===Source===
 
===Source===

Latest revision as of 05:15, 22 October 2009

{Hag AtD}


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is in BglBricks Standard. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BglBricks Standard is available at:

[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BglBricks Standard Description Page]

Source

Pool O09ig121 through O09ig128, assemble by PCA
PCR O09ig121/O09ig128 on PCA reaction        (269, EcoRI/BamHI
Sub in pBca9523-Bca1144#5                (EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-B09ig121   {?Hag AtD?}
----
O09ig121     PCA assembly of {?Hag AtD?} (B09ig121)
CCATAGAATTCATGAGATCTAACGAAAACAAAGCTAACGCTGGTATCT
O09ig122     PCA assembly of {?Hag AtD?} (B09ig121)
CCTGCGGCATAGAAGCCATAGCCATAGCAGAAGAGATACCAGCGTTAGCTTTG
O09ig123     PCA assembly of {?Hag AtD?} (B09ig121)
TATGGCTTCTATGCCGCAGGCTTATATTCCGGGTCGTTCTATGGTTACTGGCG
O09ig124     PCA assembly of {?Hag AtD?} (B09ig121)
CAACAGCACCCTGACCGTTGTGGGTAGCGATACCGCCAGTAACCATAGAACGA
O09ig125     PCA assembly of {?Hag AtD?} (B09ig121)
CAACGGTCAGGGTGCTGTTGCTGTTGGTCTGTCTAAACTGTCTGACAACGGTC
O09ig126     PCA assembly of {?Hag AtD?} (B09ig121)
TGTCAGCAGAACCGTTGATTTTGAAAACCCACTGACCGTTGTCAGACAGTTTA
O09ig127     PCA assembly of {?Hag AtD?} (B09ig121)
AATCAACGGTTCTGCTGACACTCAAGGCCATGTAGGTGCTGCTGTTGGTGCTG
O09ig128     PCA assembly of {?Hag AtD?} (B09ig121)
CGTTAGGATCCGAAGTGGAAACCAGCACCAACAGCAGCACCT

References