Difference between revisions of "Part:BBa K1886019"

 
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<partinfo>BBa_K1886019 short</partinfo>
 
<partinfo>BBa_K1886019 short</partinfo>
  
we inserted a CL protein-terminator-Pλ between Pompc and sfGFP to change the red-TCS from being activated by darkness and inhibited by red light into being activated by red light but inhibited by darkness.We also adopted in-fusion cloning to add the gene of Ho1 as well as pcyA,which play pivotal toles in both two TCS.
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we inserted a CI protein-terminator-Pλ between Pompc and sfGFP to change the red-TCS from being activated by darkness and inhibited by red light into being activated by red light but inhibited by darkness.We also adopted in-fusion cloning to add the gene of Ho1 as well as pcyA,which play pivotal toles in both two TCS.
  
  

Latest revision as of 22:24, 19 October 2016


pSB1C3_Ho1_pcyA_Pompc_CI_GFP

we inserted a CI protein-terminator-Pλ between Pompc and sfGFP to change the red-TCS from being activated by darkness and inhibited by red light into being activated by red light but inhibited by darkness.We also adopted in-fusion cloning to add the gene of Ho1 as well as pcyA,which play pivotal toles in both two TCS.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1607
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1160
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2899