Difference between revisions of "Part:BBa K1886002:Design"
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+ | Danino T, Mondragón-Palomino O, Tsimring L, et al. A synchronized quorum of genetic clocks[J]. Nature, 2010, 463(7279): 326-330. |
Latest revision as of 21:03, 19 October 2016
AHL,DPD double-cycled oscillation
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1821
Illegal BamHI site found at 4352 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 3256
Illegal AgeI site found at 4450 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1101
Illegal BsaI.rc site found at 1970
Illegal BsaI.rc site found at 3073
Illegal BsaI.rc site found at 4114
Design Notes
LAA tag has the highest degradation efficiecy in 37℃. Pay attention to the environmental temperature while using it.It can only be used in Gram-negative bacteria.
Source
From E coli genome, the subparts of this part is from iGEM Distribution kits.
References
Danino T, Mondragón-Palomino O, Tsimring L, et al. A synchronized quorum of genetic clocks[J]. Nature, 2010, 463(7279): 326-330.