Difference between revisions of "Part:BBa K1825007"

Revision as of 14:35, 18 September 2015

ZmUbi

The Zea Mays Ubiquitin promoter for expressing genes in eukariotes. However, this sequence has been shortened in the 3' because of illegal restriction sites. The original sequence has been confirmed to work in Physcomitrella patens" but this altered sequence has not. A transformation experiment in P. patens with this promoter sequence driven yellow fluorescent protein (YFP) has been carried out, but no transformants expressing YFP was observed after transformation. There is no indication that this promoter sequence works, but it is possible for other iGEM teams to use this sequence if they should want to do so.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 902
Illegal XhoI site found at 656
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 733
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1825007 short</partinfo>
-The Zea Mays Ubiquitin promoter for expressing genes in eukariotes.
+The Zea Mays Ubiquitin promoter for expressing genes in eukariotes. However, this sequence has been shortened in the 3' because of illegal restriction sites. The original sequence has been confirmed to work in ''Physcomitrella patens" but this altered sequence has not. A transformation experiment in ''P. patens'' with this promoter sequence driven yellow fluorescent protein (YFP) has been carried out, but no transformants expressing YFP was observed after transformation. There is no indication that this promoter sequence works, but it is possible for other iGEM teams to use this sequence if they should want to do so.
 <!-- Add more about the biology of this part here