Difference between revisions of "Part:BBa K1824000"

(Characterization)
(Characterization)
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===Characterization===
 
===Characterization===
[[Image:A1-J23119.png|450px]]
+
[[Image:A1-J23119.png|600px]]
  
 
===References===
 
===References===

Revision as of 11:13, 17 September 2015

Constitutive Promoter J23119 + RNA Thermometer A1

This is a composite regulatory part that consisting of constitutive promoter BBa_J23119 and RNA Thermometer A1 BBa_K1824556 for temperature induced gene expression at 42 degrees. The possible secondary structure of A1 was simulated by RNAstructure (Fig.1). This combination is compatible with the assembly method that XJTLU-CHINA promoted.


A1 RNA Thermometer.jpg
Figure 1: Possible secondary structure of RNAT A1.

Characterization

A1-J23119.png

References

1. Nocker, A. et al. mRNA-based thermosensor controls expression of rhizobial heat shock genes. Nucleic Acids Res. 29, 4800–4807 (2001).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]