Difference between revisions of "Part:BBa K1789014"
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A double-enzyme digestion test was implemented to verify this part. | A double-enzyme digestion test was implemented to verify this part. | ||
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The length of the digested DNA fits the design. | The length of the digested DNA fits the design. | ||
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Latest revision as of 15:28, 18 September 2015
SCAF2+Ter
It is the combination of scaffold system and termination.
Usage and Biology
Usage: This part is SCAF2 which is associated with a terminator. SCAF2 is used to bind TALE1 and TALE3 protein to make them fixed on the DNA. We create this part to make it more convenient every time we are required to add a scaffold.
Biology: SCAF2 is a DNA scaffold which can bind TALE protein specifically to achieve the fixing of protein or enzyme. This part is scaf2 with a terminator after it.
Sequence and Features
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 150
Illegal AgeI site found at 180
Illegal AgeI site found at 210
Illegal AgeI site found at 240
Illegal AgeI site found at 270
Illegal AgeI site found at 300
Illegal AgeI site found at 330 - 1000COMPATIBLE WITH RFC[1000]
Experimental Validation
Sequencing
This part is sequenced as correct after construction.
Enzyme digestion test
A double-enzyme digestion test was implemented to verify this part.
The length of the digested DNA fits the design.