Difference between revisions of "Part:BBa K1789013"
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A double-enzyme digestion test was implemented to verify this part. | A double-enzyme digestion test was implemented to verify this part. | ||
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The length of the digested DNA fits the design. | The length of the digested DNA fits the design. | ||
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===Functional Parameters=== | ===Functional Parameters=== | ||
Revision as of 14:43, 18 September 2015
SCAF1+Ter
This is a combination of scaffold and termination.
Usage and Biology
Usage: This part is scaf1 which is associated with a terminator. Scaf1 is used to bind TALE1 and TALE3 protein to make them fixed on the DNA. We create this part to make it more convenient every time we are required to add a scaffold.
Biology:
Scaf1 is a DNA scaffold which can bind TALE protein specifically to achieve the fixing of protein or enzyme. This part is scaf1 with a terminator after it.
Sequence and Features
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 145
Illegal AgeI site found at 175
Illegal AgeI site found at 205
Illegal AgeI site found at 235
Illegal AgeI site found at 265
Illegal AgeI site found at 295
Illegal AgeI site found at 325 - 1000COMPATIBLE WITH RFC[1000]
Experimental Validation
Sequencing
This part is sequenced as correct after construction.
Enzyme digestion test
A double-enzyme digestion test was implemented to verify this part.
The length of the digested DNA fits the design.
Functional Parameters
CHIp
Enzyme digestion test /