Difference between revisions of "Part:BBa K1680008"
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===Usage and Biology=== | ===Usage and Biology=== | ||
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===Sequence and Features=== | ===Sequence and Features=== | ||
<span class='h3bb'>This part is submitted in pSB1C3 with RFC25 standard. The part only sequence does therefore '''not''' contain a start and stop codon.</span> | <span class='h3bb'>This part is submitted in pSB1C3 with RFC25 standard. The part only sequence does therefore '''not''' contain a start and stop codon.</span> | ||
Latest revision as of 01:52, 26 September 2015
Δ1-19 Cre recombinase
This part contains the protein coding region for a mutant (Deletion of AA1-19) of DNA recombinase Cre and is codon optimized for yeast. Cre recombinases recognize two loxp sites (BBa_K1680005) and depending on their orientation cuts out or reverses the DNA sequence between them.
Sequence and Features
This part is submitted in pSB1C3 with RFC25 standard. The part only sequence does therefore not contain a start and stop codon.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 258