Difference between revisions of "Part:BBa K1615100"

 
 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1615100 short</partinfo>
 
<partinfo>BBa_K1615100 short</partinfo>
  
−
mRFP fused N terminally to CBDcipA with N and C terminal linkers.
+
mRFP (BBa_K1615089) fused to the N terminal of CBDcipA with N and C terminal linkers (BBa_K1615111). If folded properly, this allows mRFP to fluoresce while immobilised on cellulose.
 +
<br>
 +
<br>
 +
[[File:CBD RFP folding.jpg]]
 +
<br>
 +
<br>
 +
To test the RFP folding when fused to a CBD we placed 200µL of crude cell lysate in a 96 well plate and observed the fluorescence under a blue light transilluminator. This picture shows that this biobrick does fold properly.
 +
 
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 00:58, 22 September 2015

mRFP fused to Linker-CBDcipA-linker in RFC25

mRFP (BBa_K1615089) fused to the N terminal of CBDcipA with N and C terminal linkers (BBa_K1615111). If folded properly, this allows mRFP to fluoresce while immobilised on cellulose.

CBD RFP folding.jpg

To test the RFP folding when fused to a CBD we placed 200µL of crude cell lysate in a 96 well plate and observed the fluorescence under a blue light transilluminator. This picture shows that this biobrick does fold properly.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]