Difference between revisions of "Part:BBa K1615050"

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Heroin esterase (BBa_K1615045) fused to the N terminal of dCBD + N terminal linker (BBa_K1321340). If folded properly, this allows heroin esterase to deacetylate heroin while immobilised on cellulose.
 
Heroin esterase (BBa_K1615045) fused to the N terminal of dCBD + N terminal linker (BBa_K1321340). If folded properly, this allows heroin esterase to deacetylate heroin while immobilised on cellulose.
 
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[[File:Screen_Shot_2015-11-13_at_11.45.39.png]]
 
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===Usage and Biology===
 
===Usage and Biology===

Revision as of 11:46, 13 November 2015

HerE fused to Linker+dCBD in RFC25

Heroin esterase (BBa_K1615045) fused to the N terminal of dCBD + N terminal linker (BBa_K1321340). If folded properly, this allows heroin esterase to deacetylate heroin while immobilised on cellulose.
Screen Shot 2015-11-13 at 11.45.39.png Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 21
    Illegal BamHI site found at 777
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]