Difference between revisions of "Part:BBa K150000"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | AHL, a cell-cell signaling molecule is produced by the LuxI protein. The LuxI protein is expressed due to the ''luxI'' gene which is under the control of a constitutive promoter ([https://parts.igem.org/wiki/index.php?title=Part:BBa_J23107 BBa_J23107]). For this reason no input is needed for induction of gene expression. As output AHL can be measured. <br><br> | + | AHL, a cell-cell signaling molecule is produced by the LuxI protein. The LuxI protein is expressed due to the ''luxI'' gene which is under the control of a constitutive promoter ([https://parts.igem.org/wiki/index.php?title=Part:BBa_J23107 BBa_J23107]). For this reason no input is needed for induction of gene expression. As output AHL can be measuredJ23107]). For this reason no input is needed for induction of gene expression. As output AHL can be measured (see [https://parts.igem.org/Part:BBa_K150000:Experience experience] for a quantitve AHL production determination). <br><br> |
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | AHL, a cell-cell signaling molecule is produced by the LuxI protein. The LuxI protein is expressed due to the ''luxI'' gene which is under the control of a constitutive promoter ([https://parts.igem.org/wiki/index.php?title=Part:BBa_J23107 BBa_J23107]). For this reason no input is needed for induction of gene expression. As output AHL can be measured. <br> | + | AHL, a cell-cell signaling molecule is produced by the LuxI protein. The LuxI protein is expressed due to the ''luxI'' gene which is under the control of a constitutive promoter ([https://parts.igem.org/wiki/index.php?title=Part:BBa_J23107 BBa_J23107]). For this reason no input is needed for induction of gene expression. As output AHL can be measured (see [https://parts.igem.org/Part:BBa_K150000:Experience experience] for a quantitve AHL production determination). <br> |
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| − | <span class='h3bb'>Sequence and Features</span> | + | ===<span class='h3bb'>Sequence and Features</span>=== |
<partinfo>BBa_K150000 SequenceAndFeatures</partinfo> | <partinfo>BBa_K150000 SequenceAndFeatures</partinfo> | ||
Latest revision as of 14:46, 29 October 2008
3OC6HSL Sender Controlled by constitutive medium strong Promoter
This part generates the LuxI enzyme which produces AHL (3OC6HSL). It is created with a moderate strong constitutive promoter (BBa_J23107) and a 3OC6HSL (AHL) sender device (BBa_F1610).
Usage and Biology
AHL, a cell-cell signaling molecule is produced by the LuxI protein. The LuxI protein is expressed due to the luxI gene which is under the control of a constitutive promoter (BBa_J23107). For this reason no input is needed for induction of gene expression. As output AHL can be measuredJ23107]). For this reason no input is needed for induction of gene expression. As output AHL can be measured (see experience for a quantitve AHL production determination).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 698
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]