Difference between revisions of "Part:BBa K150000"

Revision as of 14:43, 29 October 2008

3OC6HSL Sender Controlled by constitutive medium strong Promoter

This part generates the LuxI enzyme which produces AHL (3OC₆HSL). It is created with a moderate strong constitutive promoter (BBa_J23107) and a 3OC₆HSL (AHL) sender device (BBa_F1610).

Usage and Biology

AHL, a cell-cell signaling molecule is produced by the LuxI protein. The LuxI protein is expressed due to the luxI gene which is under the control of a constitutive promoter (BBa_J23107). For this reason no input is needed for induction of gene expression. As output AHL can be measured.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 698
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K150000 short</partinfo>
-This part generates the LuxI enzyme which produces AHL (3OC6HSL). It is created with a moderate strong constitutive promoter (BBa_J23107) and a 3OC<sub>6</sub>HSL (AHL) sender device (BBa_F1610).
+This part generates the LuxI enzyme which produces AHL (3OC<sub>6</sub>HSL). It is created with a moderate strong constitutive promoter ([https://parts.igem.org/wiki/index.php?title=Part:BBa_J23107 BBa_J23107]) and a 3OC<sub>6</sub>HSL (AHL) sender device ([https://parts.igem.org/wiki/index.php?title=Part:BBa_F1610 BBa_F1610]).
+===Usage and Biology===
+AHL, a cell-cell signaling molecule is produced by the LuxI protein. The LuxI protein is expressed due to the ''luxI'' gene which is under the control of a constitutive promoter ([https://parts.igem.org/wiki/index.php?title=Part:BBa_J23107 BBa_J23107]). For this reason no input is needed for induction of gene expression. As output AHL can be measured. <br><br>
 <!-- Add more about the biology of this part here
 ===Usage and Biology===
-AHL, a cell-cell signaling molecule is produced by the LuxI protein. The LuxI protein is expressed due to the ''luxI'' gene which is under the control of a constitutive promoter ([https://parts.igem.org/wiki/index.php?title=Part:BBa_J23107 BBa_J23107]). For this reason no input is needed for induction of gene expression. As output AHL can be measured.
+AHL, a cell-cell signaling molecule is produced by the LuxI protein. The LuxI protein is expressed due to the ''luxI'' gene which is under the control of a constitutive promoter ([https://parts.igem.org/wiki/index.php?title=Part:BBa_J23107 BBa_J23107]). For this reason no input is needed for induction of gene expression. As output AHL can be measured. <br>
 <!-- -->