Difference between revisions of "Part:BBa K150000"
Line 1: | Line 1: | ||
− | |||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K150000 short</partinfo> | <partinfo>BBa_K150000 short</partinfo> | ||
− | This part generates the LuxI enzyme which produces AHL (3OC6HSL). | + | This part generates the LuxI enzyme which produces AHL (3OC6HSL). It is created with a moderate strong constitutive promoter (BBa_J23107) and a 3OC<sub>6</sub>HSL (AHL) sender device (BBa_F1610). |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
+ | AHL, a cell-cell signaling molecule is produced by the LuxI protein. The LuxI protein is expressed due to the ''luxI'' gene which is under the control of a constitutive promoter ([https://parts.igem.org/wiki/index.php?title=Part:BBa_J23107 BBa_J23107]). For this reason no input is needed for induction of gene expression. As output AHL can be measured. | ||
<!-- --> | <!-- --> |
Revision as of 14:41, 29 October 2008
3OC6HSL Sender Controlled by constitutive medium strong Promoter
This part generates the LuxI enzyme which produces AHL (3OC6HSL). It is created with a moderate strong constitutive promoter (BBa_J23107) and a 3OC6HSL (AHL) sender device (BBa_F1610).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 698
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]