Difference between revisions of "Part:BBa K150000"

 
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<partinfo>BBa_K150000 short</partinfo>
 
<partinfo>BBa_K150000 short</partinfo>
  
This part generates the LuxI enzyme which produces AHL (3OC6HSL).  
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This part generates the LuxI enzyme which produces AHL (3OC6HSL). It is created with a moderate strong constitutive promoter (BBa_J23107) and a 3OC<sub>6</sub>HSL (AHL) sender device (BBa_F1610).
  
 
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===Usage and Biology===
 
===Usage and Biology===
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AHL, a cell-cell signaling molecule is produced by the LuxI protein. The LuxI protein is expressed due to the ''luxI'' gene which is under the control of a constitutive promoter ([https://parts.igem.org/wiki/index.php?title=Part:BBa_J23107 BBa_J23107]). For this reason no input is needed for induction of gene expression. As output AHL can be measured.
  
 
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Revision as of 14:41, 29 October 2008

3OC6HSL Sender Controlled by constitutive medium strong Promoter

This part generates the LuxI enzyme which produces AHL (3OC6HSL). It is created with a moderate strong constitutive promoter (BBa_J23107) and a 3OC6HSL (AHL) sender device (BBa_F1610).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 698
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]