Difference between revisions of "Part:BBa K1499503"

Revision as of 01:34, 25 October 2014

quorum sensing machinery that activates GFP expression with extra terminator

This is the same part as Part:BBa_K1499500, except that it has two more terminators between the luxR gene and the luxPR promoter.

Usage and Biology

After ligating the BBa_K1499500 construct together and into the BioBrick backbone, we transformed it into the NEB 5-alpha strain of E. coli. The colonies that fluoresced were most likely to have been successfully transformed, and thus these were sent off for sequencing. The sequencing data showed that our construct was correct (see below), so we were able to submit for BioBricking.

File:Https://parts.igem.org/File:Luxoperon-cambridge2010.jpg Lux Operon

Our goal in building this construct is to create a time delay. Since the production of AHL is under the control of a lac-repressible promoter, the cascade should not begin until the promoter is induced. Additionally, once the promoter is activated, time is required for the AHL and luxR molecules to build up and interact with one another. Only after these molecules are present in large enough quantities to interact and bind to the luxPR promoter will GFP be expressed. Thus by replacing GFP with any gene of interest, this construct can be used to create a time delay for the onset of gene expression. For more information on the specifics of the time delay, see the "experience" page.

Sequence and Features