Difference between revisions of "Part:BBa K1499501:Design"

 
(→‎Design Notes)
 
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===Design Notes===
 
===Design Notes===
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Since the gene is usually secreted by the original bacterium (Neisseria Sicca), it contains a signal sequence for extra-cellular secretion. We removed this signal sequence and replaced it with a secretion tag specific for E.coli, so that the enzyme can be secreted extracellularly. In order to allow protein purification and assays, we added a His-tag at the C' terminus of the gene.  
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Since the gene is usually secreted by the original bacterium (''Neisseria Sicca''), it contains a signal sequence for extra-cellular secretion. We removed this signal sequence and replaced it with a secretion tag specific for E.coli, so that the enzyme can be secreted extracellularly. In order to allow protein purification and assays, we added a His-tag at the C' terminus of the gene.
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===Source===
 
===Source===

Latest revision as of 18:51, 2 November 2014


Endo-1,4-beta-glucanase (cellulase gene)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 313
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Since the gene is usually secreted by the original bacterium (Neisseria Sicca), it contains a signal sequence for extra-cellular secretion. We removed this signal sequence and replaced it with a secretion tag specific for E.coli, so that the enzyme can be secreted extracellularly. In order to allow protein purification and assays, we added a His-tag at the C' terminus of the gene.

Source

Genomic sequence of Neisseria Sicca SB

References