Difference between revisions of "Part:BBa K1499401:Experience"

Revision as of 00:49, 25 October 2014

To purify this protein from cell lysate, we used the New England Biolabs Chitin Magnetic Beads protein purification kit, which isolates proteins that bind to chitin-coated magnetic beads. The following gel image shows that BBa_K1499401 (nicknamed C2) was successfully purified with the chitin magnetic beads, suggesting that it exhibits chitin-binding activity. Pending cellulose-binding and waterproofing assays, C2 is a candidate for waterproofing both fungal mycelium and bacterial cellulose.

Figure 1. C1 and C2 (PdomMRNAr1.2-08705.1) after chitin magnetic bead purification. C2 was successfully purified with chitin magnetic beads, suggesting that it this protein has functional chitin-binding domains as predicted by PSI-BLAST.

Applications of BBa_K1499401

User Reviews

UNIQa627d278abaa0fb4-partinfo-00000000-QINU UNIQa627d278abaa0fb4-partinfo-00000001-QINU

@@ Line 1: / Line 1: @@
 __NOTOC__
-To purify this protein from cell lysate, we used the New England Biolabs Chitin Magnetic Beads protein purification kit, which isolates proteins that bind to chitin-coated magnetic beads. The following gel image shows that PdomMRNAr1.2-08705.1 (nicknamed C2) was successfully purified with the chitin magnetic beads, suggesting that it exhibits chitin-binding activity. Pending cellulose-binding and waterproofing assays, C2 is a candidate for waterproofing both fungal mycelium and bacterial cellulose.
+To purify this protein from cell lysate, we used the New England Biolabs Chitin Magnetic Beads protein purification kit, which isolates proteins that bind to chitin-coated magnetic beads. The following gel image shows that BBa_K1499401 (nicknamed C2) was successfully purified with the chitin magnetic beads, suggesting that it exhibits chitin-binding activity. Pending cellulose-binding and waterproofing assays, C2 is a candidate for waterproofing both fungal mycelium and bacterial cellulose.
 [[File:SBSiGEM2014_Wasp_Protein_Gel_Chitin.png|500px|thumb|center|<b>Figure 1.</b> C1 and C2 (PdomMRNAr1.2-08705.1) after chitin magnetic bead purification. C2 was successfully purified with chitin magnetic beads, suggesting that it this protein has functional chitin-binding domains as predicted by PSI-BLAST.]]