Difference between revisions of "Part:BBa K1499401:Experience"
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To purify this protein from cell lysate, we used the New England Biolabs Chitin Magnetic Beads protein purification kit, which isolates proteins that bind to chitin-coated magnetic beads. The following gel image shows that PdomMRNAr1.2-08705.1 (nicknamed C2) was successfully purified with the chitin magnetic beads, suggesting that it exhibits chitin-binding activity. Pending cellulose-binding and waterproofing assays, C2 is a candidate for waterproofing both fungal mycelium and bacterial cellulose.
 
To purify this protein from cell lysate, we used the New England Biolabs Chitin Magnetic Beads protein purification kit, which isolates proteins that bind to chitin-coated magnetic beads. The following gel image shows that PdomMRNAr1.2-08705.1 (nicknamed C2) was successfully purified with the chitin magnetic beads, suggesting that it exhibits chitin-binding activity. Pending cellulose-binding and waterproofing assays, C2 is a candidate for waterproofing both fungal mycelium and bacterial cellulose.
  
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[[File:SBSiGEM2014_Wasp_Protein_Gel_Chitin.png|500px|thumb|center|<b>Figure 2.</b> C1 and C2 (PdomMRNAr1.2-08705.1) after chitin magnetic bead purification. C2 was successfully purified with chitin magnetic beads, suggesting that it this protein has functional chitin-binding domains as predicted by PSI-BLAST.]]
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[[File:SBSiGEM2014_Wasp_Protein_Gel_Chitin.png|500px|thumb|center|<b>Figure 1.</b> C1 and C2 (PdomMRNAr1.2-08705.1) after chitin magnetic bead purification. C2 was successfully purified with chitin magnetic beads, suggesting that it this protein has functional chitin-binding domains as predicted by PSI-BLAST.]]
  
 
===Applications of BBa_K1499401===
 
===Applications of BBa_K1499401===

Revision as of 00:46, 25 October 2014


To purify this protein from cell lysate, we used the New England Biolabs Chitin Magnetic Beads protein purification kit, which isolates proteins that bind to chitin-coated magnetic beads. The following gel image shows that PdomMRNAr1.2-08705.1 (nicknamed C2) was successfully purified with the chitin magnetic beads, suggesting that it exhibits chitin-binding activity. Pending cellulose-binding and waterproofing assays, C2 is a candidate for waterproofing both fungal mycelium and bacterial cellulose.

Figure 1. C1 and C2 (PdomMRNAr1.2-08705.1) after chitin magnetic bead purification. C2 was successfully purified with chitin magnetic beads, suggesting that it this protein has functional chitin-binding domains as predicted by PSI-BLAST.

Applications of BBa_K1499401

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