Difference between revisions of "Part:BBa K1499401:Design"
 
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===Design Notes===
 
===Design Notes===
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The sequence for this gene was predicted through peptide mass fingerprinting of ''Polistes dominula'' (European paper wasp) nest protein samples. We believe it to be a potential candidate for the salivary protein that the wasps use to build and waterproof their nests. We have codon-optimized it for ''E. coli'' expression.
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The sequence for this gene was obtained from the <i>Polistes dominula</i> genome[1]. We codon-optimized it for expression in <i>E. coli</i> and ordered it for synthesis with BioBrick prefix and suffix from Integrated DNA Technologies.
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<b>Note:</b> This coding part does not contain a promoter, RBS, transcriptional terminator, etc. These elements must be added for proper functionality.
  
 
===Source===
 
===Source===
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''Polistes dominula'' genome:
 
''Polistes dominula'' genome:
 
http://goblinx.soic.indiana.edu/PdomGDB
 
http://goblinx.soic.indiana.edu/PdomGDB
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Studies of amino acid composition of nest protein in other ''Polistes'' species:
 
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http://link.springer.com/article/10.1007/PL00001733
 
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http://link.springer.com/article/10.1007/s101640070003
 
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http://link.springer.com/article/10.1007/BF00997166
 
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http://link.springer.com/article/10.1007/BF00982111
 

Latest revision as of 17:13, 24 October 2014

Chitin-binding wasp protein 2 (PdomMRNAr1.2-08705.1)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence for this gene was obtained from the Polistes dominula genome[1]. We codon-optimized it for expression in E. coli and ordered it for synthesis with BioBrick prefix and suffix from Integrated DNA Technologies.

Note: This coding part does not contain a promoter, RBS, transcriptional terminator, etc. These elements must be added for proper functionality.

Source

Polistes dominula genome.

References

Polistes dominula genome: http://goblinx.soic.indiana.edu/PdomGDB