Difference between revisions of "Part:BBa K1499400:Experience"
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To attempt to purify this protein from cell lysate, we used the New England Biolabs Chitin Magnetic Beads protein purification kit, which isolates proteins that bind to chitin-coated magnetic beads. The following gel image shows that BBa_K1499400 (nicknamed C1) was not successfully purified with the chitin magnetic beads, suggesting that it may not have the chitin-binding activity predicted by PSI-BLAST. | To attempt to purify this protein from cell lysate, we used the New England Biolabs Chitin Magnetic Beads protein purification kit, which isolates proteins that bind to chitin-coated magnetic beads. The following gel image shows that BBa_K1499400 (nicknamed C1) was not successfully purified with the chitin magnetic beads, suggesting that it may not have the chitin-binding activity predicted by PSI-BLAST. | ||
| − | [[File:SBSiGEM2014_Wasp_Protein_Gel_Chitin.png|500px|thumb|center|<b>Figure 1.</b> C1 (BBa_K1499400) and C2 (BBa_K1499401) after chitin magnetic bead purification. C2 was successfully purified with chitin magnetic beads, suggesting that this protein has functional chitin-binding domains as predicted by PSI-BLAST.]] | + | [[File:SBSiGEM2014_Wasp_Protein_Gel_Chitin.png|500px|thumb|center|<b>Figure 1.</b> C1 (BBa_K1499400) and C2 (BBa_K1499401) after chitin magnetic bead purification. Although C1 did not show up on the gel, C2 was successfully purified with chitin magnetic beads, suggesting that this protein has functional chitin-binding domains as predicted by PSI-BLAST. See the page for BBa_K1499401 for more information on C2.]] |
===Applications of BBa_K1499400=== | ===Applications of BBa_K1499400=== | ||
Latest revision as of 00:57, 25 October 2014
To attempt to purify this protein from cell lysate, we used the New England Biolabs Chitin Magnetic Beads protein purification kit, which isolates proteins that bind to chitin-coated magnetic beads. The following gel image shows that BBa_K1499400 (nicknamed C1) was not successfully purified with the chitin magnetic beads, suggesting that it may not have the chitin-binding activity predicted by PSI-BLAST.
Figure 1. C1 (BBa_K1499400) and C2 (BBa_K1499401) after chitin magnetic bead purification. Although C1 did not show up on the gel, C2 was successfully purified with chitin magnetic beads, suggesting that this protein has functional chitin-binding domains as predicted by PSI-BLAST. See the page for BBa_K1499401 for more information on C2.
Applications of BBa_K1499400
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