Difference between revisions of "Part:BBa K1499400:Design"
 
Line 17: Line 17:
 
''Polistes dominula'' genome:
 
''Polistes dominula'' genome:
 
http://goblinx.soic.indiana.edu/PdomGDB
 
http://goblinx.soic.indiana.edu/PdomGDB
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Studies of amino acid composition of nest protein in other ''Polistes'' species:
 
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http://link.springer.com/article/10.1007/PL00001733
 
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http://link.springer.com/article/10.1007/s101640070003
 
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http://link.springer.com/article/10.1007/BF00997166
 
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http://link.springer.com/article/10.1007/BF00982111
 

Latest revision as of 17:12, 24 October 2014

Chitin-binding wasp protein 1 (PdomMRNAr1.2-03231.1)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 143
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence for this gene was obtained from the Polistes dominula genome[1]. We codon-optimized it for expression in E. coli and ordered it for synthesis with BioBrick prefix and suffix from Integrated DNA Technologies.

Note: This coding part does not contain a promoter, RBS, transcriptional terminator, etc. These elements must be added for proper functionality.

Source

Polistes dominula genome.

References

Polistes dominula genome: http://goblinx.soic.indiana.edu/PdomGDB